ABSTRACT:
Type 2 Diabetes mellitus is a chronic metabolic condition known as hyperglycemia due to insulin deficiency. Dipeptidyl Peptidase -4 (DPP-4) inhibitor, or gliptin such as sitagliptin, linagliptin, vidagliptin, etc. it use as oral therapies that stabilize blood glucose by enhancing incretin hormone activity. This study evaluated different analytical method for identification and quality assurance of gliptin in bulk and pharmaceutical dosage forms. A comparative analysis of techniques, including UV-visible spectrophotometry, RP-HPLC, HPTLC, LC-MS was conducted. Each approach can show critical performance such as linearity, mobile phase, sensitivity, maximum absorbance wavelength and sensitivity. Adherence to ICH and GMP guideline is central to review, it shows the method validation to guarantee drug potency and purity. The result shows the UV-visible spectroscopy provide rapid, cost effective for basic testing, RP-HPLC remain best for routine quality control due to its exceptional precision and resolution. This synthesis offer a technical roadmap for selecting optimal platform for gliptin analysis in modern pharmaceutical environment.