ABSTRACT:
Cassia auriculata widely used traditional herb in the diabetes and skin diseases and commonly known as Tanner’s Senna. In the present study phytochemical analysis and antimicrobial activity of the flowers of the plant were investigated. The collected flowers were extracted with 80%of alcohol and tested for the presence of various phytoconstituents. The physicochemical parameters and total phenolic and total flavonoid content were determined. Thereafter the antimicrobial activity of the extract was performed. The phytochemical screening showed the presence of flavonoids, phenolics, tannins, alkaloids, glycosides, saponins, and terpenoids. TLC o analysis for flavonoids showed 2 spots with Rf value of 0.259, 0.53 and steroids showed 4 spots with Rf value of 0.228, 0.354, 0.6, 0.859. Total phenolic content was found to be 23.83µg, gallic acid equivalents and total flavonoid was 17.76µg, quercetin equivalents. The extract found to be effective against gram +ve and gram –ve bacteria with MIC against Staphylococcus aureus 6mm and and Bacillus subtilis 5mm and pseudomonas aeruginosa 8mm with 200mg concentration. Overall, the findings support the traditional use of Cassia auriculata flowers and suggest that the extract possesses significant phytochemical and antimicrobial potential.