International Journal of Research in Pharmacy and Allied Science

ISSN: 2583-6544   |  Issues per year: Monthly  |   Indexed In: Google Scholar, CORE, ScienceOpen, Researchgate

ISSN:2583-6544

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RP-HPLC Method Development and Validation on Nortriptyline Hydrochloride Tablets

Author(s): Dipak Narsing Shinde1, Dr.Balaji M. Shetkar2, Dr. Kranti L Satpute3

Email(s): 1dipakshinde7465@gmail.com

Address:

    Dayanand College of Pharmacy, Latur, Maharashtra, India..

Published In:   Volume - 2,      Issue - 4,     Year - 2023

DOI: Not Available

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ABSTRACT:
RP-HPLC method was developed for Nortriptyline Hydrochloride in the tablet dosage form. The chromatographic separation was performed on InfinityLab Poroshell 120 EC-C18 (150 mm×4.6 mm, 4 µm) column, with a mobile phase, Acetonitrile with 0.1% OPA [pH 5.0] in the ratio of 50:50 v/v at the flow rate of 1.0 ml/min. The detection was carried out at 250 nm using PDA detector. The retention time of NH was found to be 2.24 min with a run time of 10 min. As per ICH Q2R1 guideline, the method was validated for linearity, accuracy, precision and robustness. The linearity of NH was found in the range of 10-50 µg/ml. The correlation coefficient for NH was found 0.99. This demonstrates that the developed method is economical, simple, precise, rapid, selective and accurate for the estimation of NH in tablet dosage form and it can be successfully utilized for the quantitative analysis of pharmaceutical tablet dosage formulations containing Nortriptyline hydrochloride.

Keywords:

Cite this article:
Dipak Narsing Shinde, Dr.Balaji M. Shetkar, Dr. Kranti L Satpute. RP-HPLC Method Development and Validation on Nortriptyline Hydrochloride Tablets. IJRPAS, May-June 2023; 2(3): 1-9.


1.      Indian Pharmacopoeia. A government of India, Ministry of Health and Family Welfare, The Indian Pharmacopoeia Commission, Ghaziabad, 2018, II, pp 2754-2755.

2.      Bohren Y, Tessier LH, Megat S, Petitjean H, Hugel S, Daniel D, Kremer M, Fournel S, Hein L, Schlichter R, Freund-Mercier MJ, Yalcin I, Barrot M. Antidepressants suppress neuropathic pain by a peripheral β2-adrenoceptor mediated anti-TNFα mechanism. Neurobiol Dis. 2013 Dec;60:39-50. [PubMed]

3.      U.S. Pharmacopoeia 38, National formulary 33, U.S. Pharmacopoeial Convention, Vol.3, 2015, Page No.4598-4599.

4.      Tukaram B Sawant & Dhananjay V. Mane, “The Development and Validation of Stability Indicating Analytical Method for Determination of Nortriptyline in Nortriptyline HCl Tablets by Liquid Chromatography”, Indian Journal of Pharmaceutical Education and Research, 2017; Vol. 51 (4): 761-768.

5.      Suresh Jain & Disha Patel, “Development and Validation of Stability Indicating RP-HPLC Method for the Estimation of Nortriptyline Hydrochloride in Tablet Dosage Form”, International Journal Of Pharmacy & Pharmaceutical Research an official publication of Human Journal, 2018; Vol. 12 (1): 221-235.

6.      Safwan Ashourn & Nuha Kattan, “Simultaneous determination of nortriptyline hydrochloride and fluphenazine hydrochloride in microgram quantities from low dosage forms by liquid chromatography–UV detection”, Journal of Pharmaceutical Analysis, 2012;Vol.2 (6):437–442.

7.      Haritha Potluri1,Sreenivasa Rao Battula & Sunandamma Yeturu, “Validated Stability Indicating RP-HPLC Method For Simultaneous Determination Of Nortriptyline And Pregabalin In Bulk And Combined Dosage Formulations”, Journal Of The C.hilean Chemical Society, 2017; Vol. 62 (2):3490-3495.

8.      Nafisur Rahman, Habibur Rahman & Asma Khatoon, “A Validated Kinetic Method For Determination Of Nortriptyline Hydrochloride In Pharmaceutical Formulations”, International Journal Of Pharmaceutical Chemistry, 2014; V0l. 04 (03):99-105.

9.      Rajesh K. Patel , Sandip Dholakiya , Santosh Vaidya , Naitik Gohil , Dharmesh Golwala & Neha S. Mochi, “A Novel Spectrophotometric And RP-HPLC Methods For Determination Of Nortriptyline Hydrochloride And Pregabalin In Tablets”, International Journal Of Pharmaceutical Research & Allied Sciences, 2020, 9(3):1-9.

10.  V. S. Thiruvengadarajan, N. Arunkumar, Rajasekaran Aiyalu, Ponnilavarasan I & N. Tamilselvi, “Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Gabapentin and Nortriptyline Hydrochloride in Bulk and Tablet Dosage Form”, Journal of Young Pharmacist, 2022; 14(2) : 187-191.

11.  N.A. El Ragehy, S.S. Abbas & S.Z. El-khateeb, “Stability Indicating Method For Determination Of Nortriptyline Hydrochloride Using 3-methyl-2-benzothiazolinoneHydrazone (MBTH)”, Journal Of Pharmaceutical And Biomedical Analysis, 2001; Vol.25 (1) : 143–151.

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