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Author(s): Bayye Kavitha Rani ⃰1, Kedari Navya2, Mrs. N.Ashritha3

Email(s): 1kavitabayye20@gmail.com

Address:

    Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences and Technologies, JNTUK Kakinada, Andhra Pradesh- 533003

Published In:   Volume - 4,      Issue - 6,     Year - 2025

DOI: https://doi.org/10.71431/IJRPAS.2025.4612  

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ABSTRACT:
A simple, precise, accurate and reproducible method for the estimation of Berberine in bulk and polyherbal formulations by RP-HPLC. The separation of the phytochemical using RP-HPLC was achieved on a SHISEIDO CAPSELL PAK C18, (250×4.6mm, 5 micron) size column with a mobile phase consisting Acetonitrile : 0.1% Orthophosphoric acid (60:40), using a flow rate of 1 mL/min and detecting a wavelength of 265 nm.The retention times was observed to be 2.678 for Berberine respectively. Linearity was found to be 2, 4, 6, 8, 10μg/ml for Berberine, with correlation of coefficient of 0.996 respectively. For system precision the %RSD for Berberine was found to be less than 2.0. the developed method was succesfully validated for Accuracy, Precision, Linearity,Limit of Detection, Limit of Quantification & Robustness. Hence, this method can be used for estimation of Berberine in bulk and polyherbal formulations by RP-HPLC.

Cite this article:
Bayye Kavitha Rani, Kedari Navya, Mrs. N.Ashritha. A Novel RP-HPLC Analytical Method Development and Validation of Berberine In Bulk and Polyherbal Formulation. IJRPAS, June 2025; 4 (6): 108-115.DOI: https://doi.org/https://doi.org/10.71431/IJRPAS.2025.4612


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15.         Kamal, Y. T., M. Singh, E. T. Tamboli, R. Parveen, and S. Ahmad. "Quantitative analysis of berberine in Berberis aristata fruits and in a traditional anti-inflammatory unani formulation by use of a validated HPLC method." Acta Chromatographica 23, no. 1 (2011): 157-168.

16.         Pasrija, Anubhuti, Rahul Singh, and Chandra Kant Katiyar. "Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed ayurvedic dosage forms." International Journal of Ayurveda Research 1, no. 4 (2010): 243.

17.         Shigwan, Hemant, Arvind Saklani, P. D. Hamrapurkar, Tukaram Mane, and Priyanka Bhatt. "HPLC method development and validation for quantification of berberine from Berberis aristata and Berberis tinctoria." International Journal of Applied Science and Engineering 11, no. 2 (2013): 203-211.

18.         Jain, Shweta, Shalini Tripathi, and Pushpendra Kumar Tripathi. "Antiarthritic potential of berberine loaded invasomal gel." Phytomedicine Plus 2, no. 4 (2022): 100373.

19.         Deepak, Pradhan, Biswasroy Prativa, and K. A. Suri. "Isolation of berberine from Berberis vulgaris Linn. and standardization of aqueous extract by RP-HPLC." International Journal of Herbal Medicine 1, no. 2 (2013): 106-111.

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