ABSTRACT:
A rapid, accurate, simple, and reproducible UV, HPLC, and stability-indicating spectroscopic method has been developed for the quantitative estimation of Tigecycline in both bulk drug and pharmaceutical dosage forms. A selective, precise, and stability-indicating high-performance liquid chromatography (HPLC) method was established and validated for analyzing Tigecycline in its pharmaceutical formulation. The method was validated in accordance with ICH guidelines to ensure its reliability. HPLC remains the most widely adopted technique for separating, identifying, and quantifying pharmaceutical compounds. To optimize the analytical method, several chromatographic parameters were fine-tuned, including sample preparation, selection of mobile phase, choice of column, and detection system. This paper aims to present a detailed overview of the method development, optimization, and validation procedures.
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