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Author(s): Mr. Shrinivas Kapale1, Mr. Gopal Lohiya2, Dr.Kranti Satpute3

Email(s): 1kapaleshri007@gamil.com

Address:

    Dayanand Education Society’s, Dayanand College Of Pharmacy,Latur -413512, Maharashtra

Published In:   Volume - 3,      Issue - 6,     Year - 2024

DOI: Not Available

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ABSTRACT:
UV-Visible spectrophotometry is a widely employed analytical technique for the quantitative determination of various chemical substances in pharmaceutical, biological, and environmental samples. The method development process involves optimizing parameters such as wavelength selection, solvent compatibility, and calibration curve establishment to ensure accuracy, precision, and reproducibility. This study focuses on the development and validation of a UV-Visible spectrophotometric method for [specific analyte] in [specific matrix, e.g., pharmaceutical formulations]. The analytical method was developed by identifying the maximum absorption wavelength (λmax) of the analyte and optimizing the sample preparation procedure. A linear relationship was observed between absorbance and concentration within the range of [specific concentration range], with a correlation coefficient (R²) of [specific value]. Method validation was performed following ICH guidelines, assessing parameters such as linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The results demonstrated the method's robustness and suitability for routine quality control analysis. This study highlights the effectiveness of UV-Visible spectrophotometry in achieving reliable and efficient analytical solutions for various applications.

Cite this article:
Mr. Shrinivas Kapale, Mr. Gopal Lohiya, Dr.Kranti Satpute. Review on Analytical Method Development & Validation by Using UV-Visible Spectroscopy. IJRPAS, 2024; 3(6): 147-159.


1.         Hema, Swati Reddy G. A review on new analytical method development and validation by RP-HPLC. Int Res J Pharm Biosci 2017;4:41-50.

2.         Ravisankar P, Navya CN, Pravallika D, Sri DN. A review on stepby-step analytical method validation. IOSR J Pharm 2015;5:7-19.

3.         Patel A, Dwivedi N, Kaurav N, Bashani S, Patel S, Sharma HS, et al. Chemical analysis of pharmaceuticals: a review. J Med Pharm Innov 2016;3:4-7.

4.         Jatto E, Okhamafe AO. An overview of pharmaceutical validation and process controls in drug development. Trop J Pharm Res 2002;1:115-22.

5.         Pathuri R, Muthukumaran M, Krishnamoorthy B, Nishat A. A review on analytical method development and validation of the pharmaceutical technology. Curr Pharm Res 2013;3:855-70.

6.         Patil R, Deshmukh T, Patil V, Khandelwal K. Revie\w on analytical method development and validation. Res Rev J Pharm Anal 2014;3:1-10.

7.         Chauhan A, Mittu B, Chauhan P. Analytical method development and validation: a concise review. J Anal Bioanal Tech 2015;6:1.

8.         Mahar P, Verma A. Pharmaceutical process validation: an overview. Int J Pharm Res Biosci 2014;3:243-62.

9.         Lavanya G, Sunil M, Eswarudu MM, Eswaraiah MC, Harisudha K, Spandana BN, et al. Analytical method validation: an updated review. Int J Pharm Sci Res 2013;4:1280.

10.     Verma P, Madhav NS, KR Gupta V. A review article on pharmaceutical validation and process controls. Pharma Innovation 2012;1:51-60.

11.     Md Alamshoaib. Pharmaceutical process validation: an overview. J Adv Pharm Edu Res 2012;2:185-200.

12.     Ahir KB, Singh KD, Yadav SP, Patel HS, Poyahari CB. Overview of validation and basic concepts of process validation. Sch Acad J Pharm 2014;3:178-90.

13.     Nandhakumar L, Dharmamoorthy G, Rameshkumar S, Chandrasekaran S. An overview of pharmaceutical validation: quality assurance viewpoint. Int J Res Pharm Chem 2011;1:1003-14.

14.     Bhardwaj SK, Dwivedi K, Agarwal DD. A review: HPLC method development and validation. Int J Anal Bioanal Chem 2015;5:76-1.

15.     Araujo P. Key aspects of analytical method validation and linearity evaluation. J Chromatogr B 2009;877:2224-34.

16.     Goyal D, Maurya S, Verma C. Cleaning validation in the pharmaceutical industry-an overview. Pharma Tutor 2016;4:14-20.

17.     Lodhi B, Padamwar P, Patel A. Cleaning validation for the pharmaceuticals,biopharmaceuticals, cosmetic and

1.         neutraceuticals industries. J Innov Pharm Biol Sci 2014;1:27-38.

18.     Murthy DN, Chitra K. A review article on cleaning validation. Int J Pharm Sci Res 2013;4:3317.

19.     Srivastava RK, Kumar SS. An updated review: analytical method validation. Eur J Pharm Med Res 2017;4:774-84.

20.     Patel Paresh U. Development and validation of simultaneous equations method for estimation of betahistine dihydrochloride and prochlorperazine maleate in tablet dosage form. Inventi Rapid: Pharm Analysis and Quality Assurance; 2013.

21.     Nirupa G, Tripathi UM. RP-HPLC analytical method development and validation for simultaneous estimation of two drugs nitazoxanide, ofloxacin and its pharmaceutical dosage

2.         forms. Int J ChemTech Res 2012;5:775-83.

22.     Devi TP, Setti A, Srikanth S, Nallapeta S, Pawar SC, Rao JV, et al. Method development and validation of paracetamol drug by RP-HPLC. J Med Allied

23.     Nayudu ST, Suresh PV. Bio-analytical method validation–a review. Int J Pharm Chem.

24.     Daksh S, Goyal A, Pandiya CK. Validation of analytical methods– strategy and significance. Int J Res Dev Pharm Life Sci 2015;4:1489-97.

25.     Tijare LK, Rangari NT, Mahajan UN. A review on bioanalytical method development and validation. Asian J Pharm Clin Res 2016;9:6-10.

26.     Tiwari G, Tiwari R. Bioanalytical method validation: an updated review. Pharm Methods 2010;1:25-8.

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